Premium
EFFECT OF HYDROGEN PEROXIDE PRETREATMENT ON THE ENZYMATIC HYDROLYSIS OF CELLULOSE
Author(s) -
SHEN GUANGLIN,
TAO HONG,
ZHAO MOUMING,
YANG BAO,
WEN DONGQI,
YUAN QINGZHAO,
RAO GUOHUA
Publication year - 2011
Publication title -
journal of food process engineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.507
H-Index - 45
eISSN - 1745-4530
pISSN - 0145-8876
DOI - 10.1111/j.1745-4530.2009.00518.x
Subject(s) - hemicellulose , chemistry , enzymatic hydrolysis , cellulose , cellulase , lignin , hydrolysis , bioconversion , hydrogen peroxide , nuclear chemistry , reducing sugar , yield (engineering) , sugar , organic chemistry , materials science , fermentation , metallurgy
In this work, the effect of oxidation pretreatment on the enzymatic hydrolysis of cellulose in tobacco stems was evaluated. A comparison was made between the effect of H 2 O 2 pretreatment and conventional alkali pretreatment on the weight loss of cellulose, lignins and hemicelluloses of tobacco stems. The effects of H 2 O 2 concentration, pretreatment time and temperature on the enzymatic hydrolysis of tobacco stems were investigated. This pretreatment could give a higher recovery of cellulose and better removal of lignin and hemicellulose than alkali‐only pretreatment. Compared with the untreated stems, the yield of reducing sugar pretreated by H 2 O 2 pretreatment (pH 11.5, 0.6% H 2 O 2 ) for 9 h at 60C increased 347.2%. The results of scanning electron microscope and X‐ray diffraction analysis also showed that the structural and crystalline properties of the tobacco stems were changed by pretreatment, and they were in favor of the following enzymatic hydrolysis. The optimal pretreatment conditions were 60C of temperature, 0.6% of H 2 O 2 concentration and 9 h of time, which were calculated by orthogonal experiments.PRACTICAL APPLICATIONS Pretreatment on lignocellulosic materials to disrupt the recalcitrant structure is helpful for their bioconversion into low‐molecular weight substances. The tobacco stems were pretreated by H 2 O 2 oxidation before subjecting to enzymatic hydrolysis by cellulase in this work. This pretreatment was confirmed effectively to improve the recovery of celllulose, and helpful to remove lignin and hemicellulose. Moreover, the yield of reducing sugar produced by cellulase hydrolysis was apparently improved. These results were helpful for the extensive development of tobacco stems.