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EFFECT OF ENZYMATIC TREATMENT OF CORN GLUTEN MEAL ON LUTEIN, ZEAXANTHIN AND β ‐CRYTOXANTHIN EXTRACTION
Author(s) -
LI XIUXIA,
HAN LUJIA
Publication year - 2009
Publication title -
journal of food process engineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.507
H-Index - 45
eISSN - 1745-4530
pISSN - 0145-8876
DOI - 10.1111/j.1745-4530.2007.00211.x
Subject(s) - corn gluten meal , zeaxanthin , lutein , chemistry , yield (engineering) , xanthophyll , extraction (chemistry) , gluten , response surface methodology , food science , substrate (aquarium) , enzymatic hydrolysis , hydrolysis , chromatography , carotenoid , raw material , biochemistry , soybean meal , biology , organic chemistry , materials science , ecology , metallurgy
Response surface methodology was used to optimize some operating parameters on the effects of alcalase treatment extraction of lutein, zeaxanthin and β ‐cryptoxanthin from corn gluten meal (CGM) on the yield of total xanthophylls. Three independent variables tested were enzyme concentration, substrate concentration and hydrolyzing time. The t ‐test and P value indicated that the quadratic of the three variables, enzyme concentration and hydrolyzing time were important linear variables affecting the total xanthophyll yield ( P < 0.005), followed by substrate concentration ( P < 0.01). The interaction effect between enzyme concentration and hydrolyzing time was also significant ( P < 0.005). Considering the efficiency, the economy of materials and the feasibility of experiment, the technique parameters were optimized by constrained complex method. The optimum conditions were obtained as follows: the enzyme concentration, the substrate concentration and the hydrolyzing time were 4,367 U/g, 11.3% and 2.4 h, respectively. The highest total xanthophyll yield was predicted of 65.69 µ g/g when CGM was hydrolyzed under the optimum condition. The suitability of the model equation for predicting the optimal response values was tested using the selected optimum conditions; the experimental yield was 65.06 ± 0.78 µ g/g, which was found to be in agreement with the predicted yield.PRACTICAL APPLICATIONS Corn gluten meal (CGM), a major by‐product of corn wet milling, contains (on a dry basis) 200–400 µ g/g carotenoids. There are ∼840,000 tons of CGM produced in China every year. If a part of CGM produced was manufactured further to produce carotenoids as foods, materials of foods and additives of pharmaceutical products, its value and presence in the marketplace would be increased.