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PROTEOLYSIS BY IMMOBILIZED TRYPSIN
Author(s) -
GRULKE ERIC A.,
OKOS MARTIN R.,
SYVERSON ALDRICH
Publication year - 1978
Publication title -
journal of food process engineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.507
H-Index - 45
eISSN - 1745-4530
pISSN - 0145-8876
DOI - 10.1111/j.1745-4530.1978.tb00200.x
Subject(s) - autolysis (biology) , trypsin , chemistry , bovine serum albumin , chromatography , proteolysis , hydrolysis , formaldehyde , enzyme , reagent , biochemistry , organic chemistry
Trypsin immobilized on a phenol‐formaldehyde resin was used as a model system to hydrolyze bovine serum albumin. An inhibitor was used during coupling to reduce autolysis. An automated GPC column showed that the reaction product distribution of the immobilized trypsin was different from that of the soluble enzyme. Enzyme loading affected the reaction product distribution. A 5% loss in proteolytic activity was found after 31 days of use.