Premium
ANTIOXIDANT AND ANTIPROLIFERATIVE PROPERTIES OF HOT PEPPER ( CAPSICUM ANNUUM L.) SEEDS
Author(s) -
JEON GEONUK,
CHOI YOUNGMIN,
LEE SEONMI,
KIM YOUNGHWA,
OH MOONHUN,
JEONG HEONSANG,
LEE JUNSOO
Publication year - 2012
Publication title -
journal of food biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.507
H-Index - 47
eISSN - 1745-4514
pISSN - 0145-8884
DOI - 10.1111/j.1745-4514.2011.00571.x
Subject(s) - pepper , chemistry , gallic acid , antioxidant , food science , trolox , polyphenol , capsaicin , residue (chemistry) , capsicum annuum , hydroquinone , horticulture , biochemistry , dpph , biology , receptor
The antioxidant and antiproliferative activities of a methanol extract from hot pepper ( Capsicum annuum L.) seeds (pepper seed extract [PSE]) were evaluated. The polyphenolic content of the PSE was 10.9 ± 0.0 mg gallic acid equivalents/g residue, and the capsaicin and dihydrocapsaicin contents were 22.7 and 6.5 mg/100 g of residue, respectively. The 2,2‐azino‐bis‐(3‐ethylbenzothiazoline‐6‐sulfonic acid) and 1,1‐diphenyl‐2‐picrylhydrazyl radical scavenging activities of hot pepper seeds were 38.6 ± 0.7 and 20.8 ± 2.8 Trolox equivalent antioxidant capacity (mg/g residue), respectively. Pretreatment with PSE (500 µg/mL) significantly reduced ( P < 0.05) reactive oxygen species generation in HepG2 cells compared with the control; however, PSE at low concentrations (10–200 µg/mL) had no effect on cellular antioxidation. PSE showed strong antiproliferative activity against MCF7 (73.5%), MKN45 (96.8%) and HCT116 (80.0%) tumor cells at a concentration of 500 µg/mL because of an increase in apoptosis. PRACTICAL APPLICATIONS Agricultural and industrial residues are attractive sources of potential natural antioxidants. Pepper seeds are obtained as by‐products in the preparation of red pepper powder and constitute 4.14% of pepper by fresh weight. In this study, hot pepper seeds were evaluated as a new source of antioxidant and anticancer agents and as a functional material from agricultural waste. Pepper seed extract showed relatively low antioxidant activity and polyphenolic content but exerted profound antiproliferative effects on tumor cells, even at low concentration.