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ANTILISTERIAL POTENTIAL OF IMPERATORIN AND LIMONIN FROM PONCIRUS TRIFOLIATA RAFIN
Author(s) -
RAHMAN ATIQUR,
NA MINKYUN,
KANG SUN CHUL
Publication year - 2012
Publication title -
journal of food biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.507
H-Index - 47
eISSN - 1745-4514
pISSN - 0145-8884
DOI - 10.1111/j.1745-4514.2010.00528.x
Subject(s) - limonin , listeria monocytogenes , imperatorin , preservative , chemistry , bacteria , minimum inhibitory concentration , traditional medicine , biology , food science , antimicrobial , microbiology and biotechnology , chromatography , medicine , high performance liquid chromatography , genetics
To discover natural compounds with antilisterial potential, we investigated an ethyl acetate extract of the seeds of Poncirus trifoliata Rafin., and isolated imperatorin and limonin. The structures were determined on the basis of spectroscopic analysis. The compounds were tested for antibacterial activity against some Listeria monocytogenes strains and found to possess potential antilisterial activity against L. monocytogenes American Type Culture Collection (ATCC 19116, 19111, 19166, 19118 and 15313) with minimum inhibitory concentration (MIC) values ranging from 15.62 to 62.5 µg/mL. The scanning electron microscopic studies also demonstrated that imperatorin and limonin caused morphological changes of L. monocytogenes ATCC 19116 at the MIC value, along with the potential effect on cell viabilities of the tested bacteria. PRACTICAL APPLICATIONS The use of natural compounds of P. trifoliata as antilisterial agents will be suitable for applications on the food industry as natural preservatives or flavoring to control foodborne pathogens. They can be used as growth inhibitors of L. monocytogenes , the most representative foodborne pathogen. The main reason for their suitability is that usually, plant‐derived natural compounds are considered as nonphytotoxic and potentially effective against microorganisms. However, they can be toxic or harmful to humans when they are inappropriately used. Caution is generally required if natural compounds are to be taken internally or used on food commodities.