Premium
THE INHIBITORY ACTIONS OF HOUTTUYNIA CORDATA AQUEOUS EXTRACT ON DENGUE VIRUS AND DENGUE‐INFECTED CELLS
Author(s) -
LEARDKAMOLKARN VIJITTRA,
SIRIGULPANIT WIPAWAN,
PHURIMSAK CHAYAKOM,
KUMKATE SUPEECHA,
HIMAKOUN LUKANA,
SRIPANIDKULCHAI BUNGORN
Publication year - 2012
Publication title -
journal of food biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.507
H-Index - 47
eISSN - 1745-4514
pISSN - 0145-8884
DOI - 10.1111/j.1745-4514.2010.00514.x
Subject(s) - houttuynia cordata , dengue virus , dengue fever , mode of action , traditional medicine , virus , chemistry , biology , pharmacology , virology , medicine , biochemistry , chromatography , extraction (chemistry)
The antiviral activities of Houttuynia cordata ( H. cordata ) aqueous extract against dengue virus serotype 2 (DEN‐2), strain 16681, were evaluated in this study. The results showed that pre‐ and post‐incubation of H. cordata extract (10–100 µg/mL) with HepG2 cells significantly reduced intracellular DEN‐2 RNA production correlating with the decrease in dengue protein expression. In the direct blocking mode, the extract bound with DEN‐2 and strongly inhibited the intracellular viral RNA replication with an effective dose (EC 50 ) of 0.8 µg/mL. Concentrations as low as 10–40 µg/mL of H. cordata extract also exhibited protective effect on virion release from infected LLC‐MK 2 cells. High‐performance liquid chromatography analysis of H. cordata extract indicated that hyperoside was the predominant bioactive compound, and was likely to play a role in this inhibition. The extract was also shown to have no genotoxic effect on human blood cells. PRACTICAL APPLICATIONS Houttuynia cordata is recognized as medicinal vegetable consumed by people in East and Southeast Asia. It has multilateral activities in health promotion and regulation of inflammatory reactions induced by several pathogens. Dengue viral infection is a global health problem since licensed vaccines or specific antiviral drug treatments are not yet available. The current study provides scientific data to support the phytomedicinal properties of H. cordata aqueous extract on anti‐dengue virus in three modes of action (prevention, treatment and virucidal). A major constituent in the extract, hyperoside, seems to be the bioactive compound effectively acting against dengue infection. H. cordata aqueous extract was shown to be nontoxic and hyperoside may be considered a lead compound possessing drug potential for further development.