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PHENOLIC CONTENTS, ANTIOXIDANT AND TYROSINASE INHIBITORY ACTIVITIES OF LONICERA JAPONICA THUMB
Author(s) -
DUNG N.T.,
BAJPAI V.K.,
RAHMAN A.,
YOON J.I.,
KANG S.C.
Publication year - 2011
Publication title -
journal of food biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.507
H-Index - 47
eISSN - 1745-4514
pISSN - 0145-8884
DOI - 10.1111/j.1745-4514.2010.00461.x
Subject(s) - tyrosinase , antioxidant , inhibitory postsynaptic potential , japonica , chemistry , traditional medicine , food science , biochemistry , botany , biology , medicine , enzyme , neuroscience
This study aimed to evaluate the phenolics, antioxidant and tyrosinase inhibitory activities of ethanol extracts of various parts of Lonicera japonica Thumb. including flowers, leaves and branches. The ethanol extracts were found to be highly enriched in the total phenolic contents as gallic acid equivalents and in the antioxidant capacity as L‐ascorbic acid equivalents. The extracts exhibited potent 1,1‐ diphenyl‐2‐picrylhydrazyl (DPPH) and superoxide radical‐scavenging activities, which were found to be stronger than butylated hydroxyanisole and slightly weaker than ascorbic and gallic acids. The concentration of sample required to scavenge 50% of free radicals (IC 50 ) values of DPPH and superoxide radical‐scavenging capacities of the extracts were ranged between 19.45–57.97 µg/mL and 16.60–38.63 µg/mL. In addition, the extracts also exerted significant tyrosinase inhibitory activity with IC 50 values ranging from 11.16–17.18 µg/mL. The ethanol extract of L. japonica flowers displayed the highest test activities as compared with the other extracts.PRACTICAL APPLICATIONS Lonicera japonica (flowers, leaves and branches) is commonly known as traditional tea and an ingredient in traditional medicine system of several countries such as China, Korea and Vietnam. In vitro activities of the ethanol extracts of the L. japonica provide a strong antioxidant, free radical‐scavenging potential and significantly inhibited tyrosinase activities. This research indicates that all parts of L. japonica can be used to produce a natural dietary antioxidant supplement or added into healthy food products as an antibrowning agent. It can also be used as an active ingredient for antioxidant and natural skin‐whitening agent in cosmetic products. Furthermore, the antioxidant and antityrosinase‐enriched extracts of L. japonica could be the source targets for the isolation of pure antioxidants and tyrosinase inhibitors.