Premium
INDUCTION OF APOPTOSIS IN CERVIX NEOPLASMS HeLa CELLS BY A RAPESEED PEPTIDE HYDROLYSATE FRACTION
Author(s) -
XUE Z.,
YU W.,
LIU Z.,
WU M.,
WANG J.
Publication year - 2011
Publication title -
journal of food biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.507
H-Index - 47
eISSN - 1745-4514
pISSN - 0145-8884
DOI - 10.1111/j.1745-4514.2010.00451.x
Subject(s) - hela , apoptosis , dna fragmentation , hydrolysate , peptide , biology , fragmentation (computing) , programmed cell death , cell culture , microbiology and biotechnology , rapeseed , viability assay , chemistry , cell , biochemistry , food science , ecology , genetics , hydrolysis
One rapeseed peptide fraction (RSP2) was prepared from rapeseed meal by enzyme digestion and separation on Sephadex gel‐filtration column. The anticancer effects of RSP2 on human cervical HeLa cell lines were evaluated. Exposure of RSP2 caused a significant decrease in viability of HeLa cells in a dose‐dependent manner and typical morphological changes and apoptosis bleb phenomenon in RSP2‐treated HeLa cells were observed. DNA fragmentation assay and single‐cell gel electrophoresis showed that RSP2 could induce apoptosis in HeLa cells at all concentrations tested in a dose‐dependent manner. Together with our recently published data that RSP2 could decrease the percentage of HeLa cells in the G0/G1phase from 55% to 43%, and increase the ratio of apoptotic cells from 2.9 to 40%, results of this study highlighted the candidacy of RSP2 as a novel source of biologically active peptides with an inhibiting effect on human cancer cells.PRACTICAL APPLICATIONS Apoptosis is an important biological mechanism to maintain the integrity of multicellular organisms and the failure of apoptosis could lead to the development of human malignancies. Rapeseed is the world's second leading source of protein meal and could potentially be used as a food ingredient instead of being wasted or being used for animal feed. In this study, we identified one rapeseed peptide fraction prepared from rapeseed meal as a source of bioactive peptides with anticarcinogenic properties on human cancer cell through the induction of apoptosis. In addition, our previous study showed rapeseed peptide hydrolysate may be useful as a human food addition with antioxidant properties. Therefore, an underutilized by‐product such as defatted rapeseed meal could be utilized as novel therapeutic agents to fight against cancer and develop new functional foods.