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ISOLATION AND CHARACTERIZATION OF COLLAGENS FROM SCALE OF SILVER CARP ( HYPOPHTHALMICHTHYS MOLITRIX )
Author(s) -
ZHANG JUNJIE,
DUAN RUI,
YE CHAO,
KONNO KUNIHIKO
Publication year - 2010
Publication title -
journal of food biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.507
H-Index - 47
eISSN - 1745-4514
pISSN - 0145-8884
DOI - 10.1111/j.1745-4514.2010.00439.x
Subject(s) - silver carp , hypophthalmichthys , chemistry , food science , denaturation (fissile materials) , biochemistry , chromatography , fishery , biology , fish <actinopterygii> , nuclear chemistry
Acid‐soluble collagen (ASC) and pepsin‐solubilized collagen (PSC) were isolated from silver carp scales ( Hypophthalmichthys molitrix ) and some biochemical properties of scale collagens were investigated. The yields of ASC and PSC on the dry basis were 0.86% and 2.32%, respectively. Sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS‐PAGE) patterns showed that the two collagens from silver carp scale were type I collagen. They consisted of at least two subunits (α1 and α2). The molecular weight of α2 chain is approximately 116 kDa. The melting point measured by circular dichroism was 36C for scale ASC and 35.5C for scale PSC. The denaturation temperature (Td) of ASC and PSC were around 29C determined by circumvolving viscometer.PRACTICAL APPLICATIONS Collagen has been widely used in the food, pharmaceutical, cosmetic, biomedical materials and other industries. The outbreaks of bovine spongiform encephalopathy and transmissible spongiform encephalopathy have resulted in anxieties among users of cattle collagen. Fish collagen has higher safety than mammalian collagen. Collagens can be used as food casings, sausage casings, fibers or shaped products, as well as improving agent in shampoo. Silver carp is one of the major freshwater fish produced in China with the production of 3,524,800 tons in 2005. With the rapid development of fishery, some processing companies were established. A large amount of fish scales were produced, but the use of scales was very limited. This work reports on the extraction and characterization of collagens (ASC, PSC) from silver carp scale, which will offer some information for the better utilization of collagens from silver carp scale.