Premium
IDENTIFICATION OF EPIDIOXYSTEROL FROM SOUTH CHINA SEA URCHIN TRIPNEUSTES GRATILLA LINNAEUS AND ITS CYTOTOXIC ACTIVITY
Author(s) -
LIU YONGHONG,
YAN HONG,
WEN KEWEI,
ZHANG JING,
XU TUNHAI,
WANG LISHU,
ZHOU XUEFENG,
YANG XIANWEN
Publication year - 2011
Publication title -
journal of food biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.507
H-Index - 47
eISSN - 1745-4514
pISSN - 0145-8884
DOI - 10.1111/j.1745-4514.2010.00426.x
Subject(s) - cytotoxicity , sea urchin , hela , biology , in vitro , pharmacology , biochemistry , ecology
A known epidioxysterol was first isolated from South China Sea urchin Tripneustes gratilla Linnaeus, and this is the first example of epidioxysterol found in the sea urchin. The compound was evaluated for cytotoxicity against three human tumor cell lines, and showed mild cytotoxicity against SGC‐7901, HepG2 and HeLa cells with IC 50 values of 99, 65 and 94 µg/mL, respectively, while did not showed cytotoxicity against human normal hepatocytes LO2 (215 µg/mL), the result showed this peroxysterol is safe to human normal hepatocytes in the therapeutic dosages (99, 65 and 94 µg/mL) in vitro, respectively.PRACTICAL APPLICATIONS The sea urchin Tripneustes gratilla is mainly distributed in South China Sea (Huang 1984). It is an important food item in the Hainan Island. Thus, study of the metabolism of T. gratilla contributes to the understanding of its role in the natural ecosystem and is also important for homologous culture of medicine and food. There are a few chemical and biological data about T. gratilla . Only a lethal protein, heparin‐binding lectin and some toxins were isolated (Fleming and Howden 1974; Mebs 1984; Nakagawa et al . 1999). In our search for antitumor compounds from marine organisms, 5α, 8α,‐epidioxycholest‐6‐en‐3β‐ol was isolated from T. gratilla , evaluated for cytotoxicity against three tumor cell lines and showed mild cytotoxicity against SGC‐7901, HepG2 and HeLa cells, respectively, while did not showed cytotoxicity against human normal hepatocytes LO2, the result showed this peroxysterol is safe to LO2 in the therapeutic dosages (99, 65 and 94 µg/mL) in vitro , respectively.