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SINGLE‐DOSE ORAL ACUTE TOXICITY AND MUTAGENIC EFFECTS OF METHANOL EXTRACTS OF CIRSIUM JAPONICUM
Author(s) -
SHIM SOONMI,
BAE EUNKYUNG,
KIM GUNHEE
Publication year - 2011
Publication title -
journal of food biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.507
H-Index - 47
eISSN - 1745-4514
pISSN - 0145-8884
DOI - 10.1111/j.1745-4514.2010.00421.x
Subject(s) - chromosome aberration , chinese hamster , acute toxicity , toxicity , flavones , chemistry , apigenin , luteolin , ames test , pharmacology , traditional medicine , biology , salmonella , biochemistry , in vitro , flavonoid , chromosome , bacteria , genetics , medicine , chromatography , antioxidant , gene , organic chemistry
The purpose of this study was to evaluate safety on Cirsium japonicum extracts containing flavones by examining acute oral toxicity, bacterial reverse mutation and the chromosome aberration test. Mutagenicity of extracts were evaluated by a bacterial reverse mutation assay using Salmonella typhimurium TA98, TA100, TA102, TA1535, TA1537 and Escherichia coli (WP2uvrA) with and without metabolic activation. An in vitro chromosome aberration test in Chinese hamster lung cells was conducted at the concentrations of 0, 1,250, 2,500 and 5,000 µg/mL. No significant adverse effects on body weight, mortality and gross findings were observed for 15 days after a single dose (2,000 mg of extracts/kg body weight) of administration. In the bacterial reverse mutation assay, C. japonicum extracts ranging from 156 to 5,000 µg/plate did not induce base‐pair substitution or frame shift. Any significant increase in structural aberration as well as reduction of cell cycle progression was not observed in the chromosome.PRACTICAL APPLICATIONS Cirsium japonicum extracts are being developed as functional ingredients in dietary supplements due to their biological activities. However, studies on their safety evaluation are limited. In this study, we revealed that methanol extracts of C. japonicum including apigenin and luteolin did not induce acute toxicity and mutagenicity. All of the results suggest that methanol extracts of C. japonicum may be useful substances for application of dietary supplements.