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CYTOTOXIC ACTIVITIES OF PHLORETHOL AND FUCOPHLORETHOL DERIVATIVES ISOLATED FROM LAMINARIACEAE ECKLONIA CAVA
Author(s) -
LI YONG,
QIAN ZHONGJI,
KIM MOONMOO,
KIM SEKWON
Publication year - 2011
Publication title -
journal of food biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.507
H-Index - 47
eISSN - 1745-4514
pISSN - 0145-8884
DOI - 10.1111/j.1745-4514.2010.00387.x
Subject(s) - hela , brown algae , chemistry , polyphenol , antiprotozoal , antioxidant , cytotoxic t cell , biochemistry , biology , traditional medicine , algae , botany , cell , medicine , in vitro
Ecklonia cava (EC) is a member of the family Laminariaceae, and it is an edible marine brown alga with various bioactivities. Five phlorotannin derivatives from the methanol extract of Ecklonia cava were isolated and described as fucodiphloroethol G (1) with phenylether bridges (phlorethol), and dieckol (2), 1‐(3′,5′‐dihydroxyphenoxy)‐7‐(2″,4″,6″‐trihydroxyphenoxy)‐2,4,9‐trihydroxy‐dibenzo‐1,4‐dioxin (3), eckol (4), and phlorofucofurofuroeckol A (5) with biaryl linkages (fucophlorethols), their structures were identified mainly by means of nuclear magnetic resonance spectral analysis. Compound 1 was obtained and characterized for the first time from this genus, and all proton resonances of 1 for phenolic hydroxyl groups were assigned completely as well, in addition, related bioactivity reports of 1 are not found anywhere so far. In this publication, cytotoxic action and future directions of these pholorotannins were evaluated first using four cultured human tumor cell lines (HeLa, HT1080, A549 and HT‐29) and one cultured human normal cell line (MRC‐5), with suggested explanation of the structure–activity relationship.PRACTICAL APPLICATIONS The main bioactive components in marine brown algae are polyphenol derivatives with unique chemical structures, namely phlorotannins. To date, a number of bioactivities have been explored using crude extracts containing phlorotannins and/or single phlorotannins from edible marine brown alga‐ Ecklonia cava such as anti‐HIV, antioxidant, antiallergy, anti‐plasmin, anti‐inflammatory, antidiabetic, antiproliferative and anticancer activities. This report focuses on the cytotoxic activities of these pholorotannins, first using four cultured human tumor cell lines (HeLa, HT1080, A549 and HT‐29) and one cultured human normal cell line (MRC‐5), and with the suggested structure–activity relationship. The results indicate that these phlorotaninns possess cytotoxic activity at certain concentrations. Meanwhile, it provides experimental evidence for the further exploration of these compounds.