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THE PURIFICATION AND ANTIOXIDATIVE ACTIVITIES IN D‐GALACTOSE‐INDUCED AGING MICE OF A WATER‐SOLUBLE POLYSACCHARIDE FROM CORDYCEPS GUNNII (BERK.) BERK. MYCELIUM
Author(s) -
ZHU ZHENYUAN,
SI CHUANLING,
ZHONG YUERU,
ZHU CHANGMEI,
ZHOU JIAPING,
LIU ANJUN,
ZHANG YONGMIN
Publication year - 2011
Publication title -
journal of food biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.507
H-Index - 47
eISSN - 1745-4514
pISSN - 0145-8884
DOI - 10.1111/j.1745-4514.2010.00383.x
Subject(s) - cordyceps , sephadex , mycelium , galactose , polysaccharide , chemistry , monosaccharide , xylose , sorbose , antioxidant , size exclusion chromatography , biochemistry , chromatography , food science , fermentation , biology , fructose , botany , enzyme
Cordyceps sinensis is a well‐known tonic food or invigorant with broad‐spectrum medicinal properties that is widely used in China. Cordyceps gunnii (berk.) Berk is also well known as the Chinese rare caterpillar fungus and has similar pharmacological activities with C. sinensis. The water‐soluble polysaccharide CPS50‐I was extracted from the mycelia of C. gunnii and further purified by diethylaminoethyl ‐Sephadex A‐25 and Sephadex G‐75. Its characteristics were determined by chemical analysis, gas chromatography, high‐performance size‐exclusion chromatography and infrared spectroscopy. The results showed that CPS50‐I is a white powder containing 94.57% carbohydrate which is composed of four kinds of monosaccharides including xylose, mannose, glucose and galactose with a molar ratio of 0.13:0.89:0.54:1. CPS50‐I had a molecular weight of ∼9874 Da and [α] D 20 = + 85 (c 0.5, H 2 O). The protective effect of CPS50‐I against oxidation resistance in D‐galactose (D‐gal)‐induced aging mice was investigated. The results showed that CPS50‐I had a significant protective effect against D‐gal‐induced aging mice.PRACTICAL APPLICATIONS In this work, a water‐soluble polysaccharide CPS50‐I was extracted from the mycelia of Cordyceps gunnii and further purified by diethylaminoethyl‐sephadex A‐25 and sephadex G‐75. For antioxidant testing in vivo, the protective effect of CPS50‐I was investigated against oxidation resistance in D‐galactose (D‐gal)‐induced aging mice. It showed that CPS50‐I had an obviously protective effect against D‐gal‐induced aging mice. The results suggest that the water‐soluble polysaccharide CPS50‐I provide a source of natural antioxidants with potential value for health foods and therapeutics and may contribute to be used as a dietary supplement to delay the process of aging.