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ANTIDIABETES PROPERTIES OF n ‐HEXANE AND PETROLEUM ETHER EXTRACTS FROM SEEDS OF NIGELLA GLANDULIFERA FREYN
Author(s) -
XIN XUELEI,
WANG QI,
AISA HAJI AKBER,
WANG HANQING
Publication year - 2010
Publication title -
journal of food biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.507
H-Index - 47
eISSN - 1745-4514
pISSN - 0145-8884
DOI - 10.1111/j.1745-4514.2009.00318.x
Subject(s) - petroleum ether , chemistry , ranunculaceae , traditional medicine , biochemistry , biology , botany , chromatography , extraction (chemistry) , medicine
Nigella glandulifera Freyn, an annual plant species of the family Ranunculaceae, served as a traditional Uigur medicine and food. Many researchers have reported that Nigella sativa L., which is the same genus with it, has an antidiabetic effect, the seeds oil was an activity component used as a kind of function food. In the present study, the difference of oil extract between n‐hexane and petroleum ether respectively from the seeds of Nigella glandulifera Freyn were analyzed by gas chromatography‐mass spectrometry, all of them can inhibit protein tyrosine phosphatase 1B (PTP1B) with half maximal inhibitory concentration values of 33.15 ± 0.77 and 18.50 ± 0.52 µg/mL, respectively. They have different binding sites with PTP1B. Lastly, inhibited ability of PTP1B and that of TCPTP were compared; all of them have shown some selectivity.PRACTICAL APPLICATIONS In this study antidiabetic‐relevant potential in seeds of Nigella glandulifera Freyn were confirmed using in vitro enzyme assays for protein tyrosine phosphatase inhibitory activities. In vitro inhibitory activity of this enzyme provides a strong biochemical rationale for further in vivo studies and dietary management strategy for type 2 diabetes. Theses extract especially petroleum ether extract maybe generate a whole food profile that has the potential to reduce type 2 diabetes‐induced pathogenesis.