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ESR SPECTROSCOPY INVESTIGATION OF ANTIOXIDANT ACTIVITY AND PROTECTIVE EFFECT ON HYDROXYL RADICAL‐INDUCED DNA DAMAGE OF ENZYMATIC EXTRACTS FROM PICRORRHIZA KURROA
Author(s) -
CHOI SOUNGHEE,
KIM EUNKYUNG,
LEE SEUNGJAE,
JEON YOUJIN,
MOON SANGHO,
LEE CHIHO,
JEON BYONGTAE,
PARK INSHIK,
PARK TAEKYU,
KIM BOKYUNG,
PARK SEUNG HWA,
PARK PYOJAM
Publication year - 2008
Publication title -
journal of food biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.507
H-Index - 47
eISSN - 1745-4514
pISSN - 0145-8884
DOI - 10.1111/j.1745-4514.2008.00192.x
Subject(s) - chemistry , carbohydrase , dpph , antioxidant , hydroxyl radical , trypsin , enzyme , ic50 , protease , chromatography , biochemistry , in vitro
The potential antioxidant activity of enzymatic extracts from Picrorrhiza kurroa was evaluated on 2,2‐diphenyl‐1‐picrylhydrazyl (DPPH) radical, hydroxyl radical and alkyl radical‐scavenging activities using an electron spin resonance spectrometer (JEOL Ltd., Tokyo, Japan). P. kurroa was enzymatically hydrolyzed by seven carbohydrases and five proteases to prepare water‐soluble extracts. The DPPH radical‐scavenging activities of the pancreatic trypsin and Amyloglucosidase (AMG) (artificial carbohydrase by Novozyme Nordisk, Bagsvaerd, Denmark) extracts from P. kurroa were the highest among protease and carbohydrase extracts, and 50% inhibitory concentration (IC 50 ) values were 35.58 and 29.03 µg/mL, respectively. The hydroxyl radical‐scavenging activity of the Protamex and Viscozyme extracts from P. kurroa were the highest scavenging activities, and the IC 50 values were 0.46 and 1.89 mg/mL, respectively. In addition, the Protamex and Maltogenase extracts from P. kurroa showed the highest alkyl radical‐scavenging activities, and the IC 50 values were 18.03 and 10.66 µg/mL, respectively. The protective effect of the Protamex extracts from P. kurroa on DNA damage which was free radical‐induced was 92% at 3 mg/mL. These results indicate that enzymatic extracts of P. kurroa show potent antioxidant activity.PRACTICAL APPLICATIONS Picrorrhiza kurroa could be used to produce protein and carbohydrate extracts with antioxidative activity. Many industrial commercial enzymes such as Promozyme, Celluclast 1.5 L FG, Maltogenase L, Viscozyme L, Termamyl SC, Dextrozyme E, AMG 300 L, Protamex, Flavourzyme 500 MG, Neutrase 0.8 L, Pancreatic Trypsin and Alcalase 2.4 L could be also used to attain the extracts processing the high antioxidative activity. The extracts can be used as natural antioxidants.