Premium
AN IN SITU METHOD TO STUDY THE REACTION CATALYZED BY ALKALINE PHOSPHATASE ON DNPP
Author(s) -
LOPEZ ELIZABETH CONTRERAS,
CHAMPION DOMINIQUE,
ORDAZ JUDITH JAIMEZ,
MESTE MARTINE LE
Publication year - 2006
Publication title -
journal of food biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.507
H-Index - 47
eISSN - 1745-4514
pISSN - 0145-8884
DOI - 10.1111/j.1745-4514.2006.00065.x
Subject(s) - dextran , sucrose , chemistry , alkaline phosphatase , catalysis , agar , reaction rate , viscosity , polysaccharide , enzyme catalysis , enzyme , phosphate , nuclear chemistry , chromatography , biochemistry , thermodynamics , biology , bacteria , genetics , physics
The reaction catalyzed by alkaline phosphatase on disodium‐p‐nitrophenyl phosphate was studied in a reaction media composed of sucrose (43.5 and 53.5%, w/w) and sucrose (43.5%, w/w) + polysaccharide (10%, w/w) added with agar‐agar (0.5%, w/w) at low temperatures (20, 0 and − 5C). The results show that temperature and concentration of sucrose and sucrose + dextran in the media affect the enzymatic reaction. The presence of 10% dextran (molecular weight of 10 4 g/mol, w/w) in the reaction medium decreased the reaction rate. This could be explained by the increase in the viscosity of the medium, due to the presence of this polysaccharide. The experimental data were compared with predicted values, calculated from Atkin's theory. The results show that in our experimental conditions described previously, the reaction catalyzed by alkaline phosphatase cannot be predicted by Atkin's theory, probably because the viscosity was not the main parameter governing the reaction.