Premium
PURIFICATION AND PARTIAL CHARACTERIZATION OF AN ENDO‐POLYGALACTURONASE FROM ASPERGILLUS NIGER
Author(s) -
GUO CHUNTENG,
XUE WEIMING,
CHEN TIANBAO,
WENHAN DENG,
RAO PINGFAN
Publication year - 2002
Publication title -
journal of food biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.507
H-Index - 47
eISSN - 1745-4514
pISSN - 0145-8884
DOI - 10.1111/j.1745-4514.2002.tb00855.x
Subject(s) - aspergillus niger , pectinase , ammonium sulfate precipitation , chemistry , chromatography , ammonium sulfate , enzyme , ammonium , size exclusion chromatography , biochemistry , organic chemistry
A pectinase was identified and isolated from a commercial Aspergillus niger pectinase preparation. The crude enzyme preparation, which was prepared by precipitation of the water extract of the culture of A. niger with ammonium sulfate, was further fractionated by three steps of chromatography, i. e., cation exchange, hydrophobic interaction and onion exchange, to obtain an electrophoretically homogeneous pectinase. The molecular weight of the purified enzyme was estimated by SDS‐PAGE to be about 40.4 kDa under both nonreducing and reducing conditions, with the optimum pH at 5.0 and the optimum temperature at 36C. The enzyme was stable at temperatures below 35C. The partial N‐terminal ammo acid sequence data analysis of the first 19 amina acids of the obtained pectinase revealed 94.7% and 89.5% homology with two reported pectinases from A. niger.