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PHENOLIC COMPOUNDS IN THE FLESH OF KOREAN APPLE CULTIVAR, BUSA
Author(s) -
WHANG HEAJEUNG,
PARK KIHWAN,
YOON KWANGRO
Publication year - 2002
Publication title -
journal of food biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.507
H-Index - 47
eISSN - 1745-4514
pISSN - 0145-8884
DOI - 10.1111/j.1745-4514.2002.tb00769.x
Subject(s) - chemistry , protocatechuic acid , rutin , chromatography , chlorogenic acid , sephadex , p coumaric acid , quercetin , cultivar , high performance liquid chromatography , ferulic acid , food science , caffeic acid , botany , organic chemistry , biology , antioxidant , enzyme
Phenolic compounds were purified from Busa, the most widely cultivated apple cultivar in Korea, by a sequential separation employing PVPP, Ambertite XAD‐2 and Sephadex LH‐20 column chromatography. Phenolic compounds in some fractions were farther identified by directly comparing with corresponding phenolic standards after separation on HPLC and GC‐MS. The phenolic compounds identified were p‐hydoxybenzoic, protocatechuic, 4‐hydroxymetyl‐benzonic, caffeic, p‐coumaric, o‐coumaric, ferulic, sinapic and chlorogenic acids and (±)‐catechin. Among them, 4‐hydroxymethylbenzoic, protocatechuic, o‐coumaric and sinapic acids were ascertained as new members of a family of phenolic acids present‐in apples. Also the presence of rutin, quercetin and phlorizin in apples were demonstrated using HPLC.