Premium
EVALUATION OF DIFFERENT METHODS TO ISOLATE COD ( GADUS MORHUA ) MUSCLE MYOSIN
Author(s) -
KRISTINSSON HORDUR G.
Publication year - 2001
Publication title -
journal of food biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.507
H-Index - 47
eISSN - 1745-4514
pISSN - 0145-8884
DOI - 10.1111/j.1745-4514.2001.tb00737.x
Subject(s) - myosin , tropomyosin , gadus , chemistry , actin , chromatography , biochemistry , biology , fish <actinopterygii> , fishery
Four methods developed for fish muscle myosin isolation were evaluated for their potential to isolate myosin from cod white muscle. A slightly modified version of the method by Martone and coworkers (Martone et al. 1986) gave outstanding myosin purity (97.3%) as determined by SDS‐PAGE and densito‐metric analysis without employing time consuming chromalographic separation, while the other three methods tested gave myosin purity ranging from 33.4–76.75% with substantial contamination of actin and tropomyosin. The method of Manone and coworkers (1986) with more complete muscle tissue disintegration and prolonged extraction steps than used in the original work is therefore highly recommended far researchers interested in studying functionality of a pure cod myosin preparation.