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ALKALINE PHOSPHATASE FROM JAW ALA SHRIMP ( ACETES INDICUS )
Author(s) -
CHANDER RAMESH,
THOMAS PAUL
Publication year - 2001
Publication title -
journal of food biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.507
H-Index - 47
eISSN - 1745-4514
pISSN - 0145-8884
DOI - 10.1111/j.1745-4514.2001.tb00726.x
Subject(s) - hepatopancreas , alkaline phosphatase , shrimp , enzyme , phosphate , chemistry , biochemistry , phosphatase , enzyme assay , biology , fishery
Alkaline phosphatase was isolated from Jawala shrimps (Acetes indicus), a tiny crustacean. The hepatopancreas (head region) is a good source of this enzyme. pH optima of the partial purified enzyme was found to he 9.5 and optimal temperature for maximal activity was 40C. Jawala phosphatase was completely inhibited by 1, 10‐phenanthroline and EDTA, indicating that it is a metalloprotein. The activity of inhibited enzyme was restored by Zn 2+ and Mn 2+ salts. Feed back inhibition of the enzyme by inorganic phosphate was also observed.