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2,4,5‐TRIHYDROXYBUTYROPHENONE (2,4,5‐THBP) AS A SUBSTRATE FOR HORSERADISH PEROXIDASE 1
Author(s) -
KAHN VARDA,
ZAKIN VARDA
Publication year - 1996
Publication title -
journal of food biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.507
H-Index - 47
eISSN - 1745-4514
pISSN - 0145-8884
DOI - 10.1111/j.1745-4514.1996.tb00575.x
Subject(s) - horseradish peroxidase , hydrogen peroxide , substrate (aquarium) , chemistry , peroxidase , quinone , photochemistry , stereochemistry , enzyme , biochemistry , biology , ecology
2,4,5‐trihydroxybutyrophenone (2,4,5‐THBP), in the presence of hydrogen peroxide (H 2 O 2 ), is a substrate for horseradish peroxidase (HRP) with a Km value of 2.5 mM. An intermediate red product(s), probably 2,4,5‐THBP quinone, characterized by a peak at 490–500 nm, was detected and the time course of its conversion to the final red product(s) was studied. The relationships between the rate of 2,4,5‐THBP oxidation to pigmented product(s) as a function of various concentrations of H 2 O 2 , 2,4,5‐THBP and HPR are described .