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GENERATION AND APPLICATION OF IMMUNOLOGICAL PROBES FOR THE STUDY OF MEMBRANE‐BOUND PROTEINS AND ENZYMES—A REVIEW
Author(s) -
BARONE LUCILLE M.,
WASSERMAN BRUCE P.
Publication year - 1996
Publication title -
journal of food biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.507
H-Index - 47
eISSN - 1745-4514
pISSN - 0145-8884
DOI - 10.1111/j.1745-4514.1996.tb00550.x
Subject(s) - antigenicity , polyclonal antibodies , membrane protein , immunoprecipitation , enzyme , cloning (programming) , biochemistry , immunocytochemistry , function (biology) , integral membrane protein , membrane , computational biology , antibody , biology , chemistry , microbiology and biotechnology , gene , computer science , genetics , programming language , endocrinology
Immunological techniques are widely used for the detection of enzymes in food systems and for elucidating protein structure and function. The generation of anti‐bodies recognizing membrane‐bound proteins is difficult due to their hydrophobic nature, and reduced antigenicity. This review summarizes strategies for the production and purification of polyclonal antibodies recognizing membrane proteins. Applications such as enzyme immunoprecipitation, use of site‐specific anti‐peptide. antibodies for determination of the topology of membrane‐embedded proteins, immunocytochemistry, and expression vector cloning are described.