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THE USE OF SORGHUM ( SORGHUM BICOLOR L. MOENCH) DHURRINASE‐ENRICHED PREPARATION IN THE DETERMINATION OF TOTAL CYANIDE IN SORGHUM AND SORGHUM PRODUCTS
Author(s) -
IKEDIOBI CHRISTOPHER O.,
TERRY DORIS E.,
UKOHA AGWU I.
Publication year - 1994
Publication title -
journal of food biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.507
H-Index - 47
eISSN - 1745-4514
pISSN - 0145-8884
DOI - 10.1111/j.1745-4514.1994.tb00486.x
Subject(s) - sorghum , cyanide , chemistry , hydrolysis , chromatography , cellulose , acetone , sweet sorghum , enzymatic hydrolysis , adsorption , biochemistry , organic chemistry , agronomy , biology
A dhurrinase (sorghum β‐glucosidase II) which catalyzes the hydrolysis of dhurrin (p‐hydroxy‐L‐mandelonitrile β‐D‐glucoside) was purified 87‐fold from sorghum seeds using acetone precipitation, bentonite adsorption and column chromatography on hydroxyapatite and DEAE‐cellulose. Dhurrin, the natural substrate for this β‐glucosidase, was used for enzyme assay. Dhurrinase, as opposed to alkali hydrolysis of dhurrin for cyanide assay, proved to be more efficient in releasing free assayable cyanide (CN ‐ ) during the determination of total cyanide in sorghum and sorghum‐derived products.