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PREPARATION, PURIFICATION AND IDENTIFICATION OF 10‐OXO‐ TRANS ‐8‐DECENOIC ACID FROM THE CULTIVATED MUSHROOM, AGARICUS BISPORUS
Author(s) -
MAU JENGLEUN,
BEELMAN ROBERT B.,
ZIEGLER GREGORY R.
Publication year - 1992
Publication title -
journal of food biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.507
H-Index - 47
eISSN - 1745-4514
pISSN - 0145-8884
DOI - 10.1111/j.1745-4514.1992.tb00460.x
Subject(s) - chemistry , agaricus bisporus , chromatography , mushroom , ethyl acetate , chloroform , acetone , column chromatography , ether , organic chemistry , food science
A procedure was developed to isolate 10‐oxo‐trans‐8‐decenoic acid (ODA) from mushrooms. ODA was produced by homogenizing mushrooms in phosphate buffer with added linoleic acid, extracted from the supernatant after centrifugation, and purified using column and thin‐layer chromatography. The purified compound was then characterized using ultraviolet, infrared and mass spectrometry, and nuclear magnetic resonance spectroscopy.The purified compound, containing 97.5% ODA, was a white, waxy solid with a pK a of 4.68. ODA was soluble in acetone, chloroform, ethanol, ethyl ether, methanol, methylene chloride and water, and slightly soluble in pentane, hexane, heptane and benzene. The TBA test was found to be a viable method for the quantification of ODA.