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CHANGES IN THE ACTIVITY AND SUBCELLULAR DISTRIBUTION OF PPO IN ‘DELICIOUS’ APPLES DURING CONTROLLED ATMOSPHERE STORAGE
Author(s) -
BARRETT DIANE M.,
LEE C. Y.,
LIU FRANK W.
Publication year - 1991
Publication title -
journal of food biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.507
H-Index - 47
eISSN - 1745-4514
pISSN - 0145-8884
DOI - 10.1111/j.1745-4514.1991.tb00154.x
Subject(s) - polyphenol oxidase , chemistry , controlled atmosphere , modified atmosphere , food science , polyacrylamide gel electrophoresis , enzyme assay , catechol oxidase , botany , shelf life , enzyme , horticulture , biochemistry , biology , peroxidase , organic chemistry
Polyphenol oxidase (PPO) activity in 4, 000 × g, 100, 000 × g, 200 × g and soluble fractions of ‘Delicious’ apples was monitored during 28 weeks of controlled atmosphere storage. PPO activity in 4, 000 × g and 100, 000 × g fractions decreased, while that in soluble and 200 × g fractions increased. These shifts in subcellular location occurred sooner in apples which were stored under high CO 2 conditions (2.5–6% O 2 , 8–12% CO 2 ) than those under normal CA conditions (2% O 2 , 3% CO 2 ). Polyacrylamide gel electrophoresis of 4, 000 × g fractions isolated from ‘Delicious’ apples at harvest showed 2 bands with PPO activity, while stored samples all had 3 bands. Isoenzyme patterns were found to vary in different subcellular fractions.