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2, 4, 5‐TRIHYDROXYBUTYROPHENONE (2, 4, 5‐THBP) a AS A SUBSTRATE FOR MUSHROOM TYROSINASE b
Author(s) -
KAHN VARDA,
OSUGA DAVID T.,
WHITAKER JOHN R.
Publication year - 1991
Publication title -
journal of food biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.507
H-Index - 47
eISSN - 1745-4514
pISSN - 0145-8884
DOI - 10.1111/j.1745-4514.1991.tb00140.x
Subject(s) - mushroom , tyrosinase , substrate (aquarium) , chemistry , food science , biochemistry , biology , enzyme , ecology
2, 4, 5‐THBP (2, 4, 5‐trihydroxybutyrophenone) is a substrate for mushroom tyrosinase with a K m value of 1.00 mM. The final product(s) formed is red (λ. max 480–490 nm) and it is rather stable with time. An intermediate red product(s), having a maximum at λ 505–515 nm, was detected and the time course of its conversion to the final red product(s) was studied. The data suggest that the intermediate red product(s) is 2, 4, 5‐THBP‐quinone which is converted to colorless polymerized 2, 4, 5‐THBP‐OH, which is then oxidized to a red polymerized 2, 4, 5‐THBP‐quinone(s), which is the final red product(s).