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TWO‐DIMENSIONAL ELECTROPHORETIC ANALYSIS OF ENDOPOLYGALACTURONASES PRODUCED BY KLUYVEROMYCES MARXIANUS
Author(s) -
SMITH ABRAHAM B.,
PYLE DAVID L.
Publication year - 1990
Publication title -
journal of food biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.507
H-Index - 47
eISSN - 1745-4514
pISSN - 0145-8884
DOI - 10.1111/j.1745-4514.1990.tb00840.x
Subject(s) - kluyveromyces marxianus , isozyme , population , kluyveromyces , staining , electrophoresis , enzyme , biochemistry , biology , chemistry , silver stain , chromatography , microbiology and biotechnology , yeast , genetics , saccharomyces cerevisiae , demography , sociology
The high resolution technique of two‐dimensional electrophoresis is used to establish the endopolygalacturonase isoenzyme profile of Kluyveromyces marxianus, strain NCYC 587. We show that the use of this technique in nondenaturing conditions represents a powerful procedure for resolving the isoenzyme population and detecting their endopolygalacturonase activities. The enzyme staining method described is at least as sensitive as silver staining. The purified enzyme was shown to consist of nine isoenzymes. Their pIs ranged from 5.6 to 6.5 and they appeared to have similar molecular weights in the region of 43,000 daltons.