Premium
QUANTIFICATION AND SOME CHEMICAL AND BIOCHEMICAL CHARACTERIZATION OF NITROGENOUS SUBSTANCES FROM VARIETIES OF COMMON BEANS (PHASEOLUS VULGARIS, L.).
Author(s) -
SGARBIERI VALDEMIRO CARLOS,
GALEAZZI MARIA ANTONIA MARTINS
Publication year - 1990
Publication title -
journal of food biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.507
H-Index - 47
eISSN - 1745-4514
pISSN - 0145-8884
DOI - 10.1111/j.1745-4514.1990.tb00836.x
Subject(s) - phaseolus , globulin , chymotrypsin , trypsin , albumin , chemistry , fraction (chemistry) , titer , methionine , biochemistry , chromatography , enzyme , biology , botany , antibody , amino acid , immunology
Crude protein, extracted protein, and nonprotein nitrogenous substances were quantified in 55 varieties of common dry beans. Crude protein (% N x 6.25) on a dry basis varied from 23.01 to 35.22%, with a mean value of 28.51%. Methionine varied from 0.40 to 1.73% of the crude protein with a mean value of 0.80%. The ratio of globulin/albumin ranged from 1.00 to 3.31. Thirty percent of the total nitrogen was of low MW and was lost in the dialysis (MW cut off 8,000 daltons). Electrophoretic patterns (PAGE) revealed from 3 to 11 bands in the globulin fraction and 4 to 11 in the albumin fraction. Trypsin and chymotrypsin inhibitory activity was much higher in the albumin fraction than in the globulin fraction. The globulin fraction from most of the varieties was more inhibitory against trypsin than chymotrypsin. On the other hand, in the albumin fraction of the majority of the varieties the activity was stronger against chymotrypsin. Hemagglutination titer for 57 varieties ranged from 1024 to 32. Eight varieties had titers of 1024 and only four had titers of 32.