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SELECTED p ‐NITROANILIDES AS SUBSTRATES FOR SENSITIVE PAPAIN ASSAY
Author(s) -
FUKAL LADISLAV,
KASAFIREK EVZEN,
STREJCEK FRANTISEK,
KAS JAN
Publication year - 1987
Publication title -
journal of food biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.507
H-Index - 47
eISSN - 1745-4514
pISSN - 0145-8884
DOI - 10.1111/j.1745-4514.1987.tb00115.x
Subject(s) - papain , chemistry , trypsin , enzyme kinetics , bromelain , kinetics , detection limit , enzyme , oligopeptide , chromatography , stereochemistry , biochemistry , peptide , active site , physics , quantum mechanics
N‐acetyl‐Leu‐Leu‐Gly‐p‐nitroanilide and N‐succinyl‐Ala‐Ala‐(S‐benzyl) Cys‐p‐nitroanilide were found to be very sensitive papain substrates, making it possible to detect 0.5 or 0.7 μg of technical papain in 1 mL of beer in a short time. This detection limit is about 10 times lower than the usual papain level used for chillproofing. The estimated kinetic constants (k cat , K m ) for papain, bromelain and trypsin permit certain conclusions about papain specificity for oligopeptides, which will help to prepare even more specific p‐nitroanilide substrates.