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DETERMINATION OF REACTION ORDER BY LINEARIZATION AND ITS APPLICATION TO ESTIMATION OF MICHAELIS‐MENTON PARAMETERS
Author(s) -
DURANCE T. D.,
MAKHIJANI B. M.,
SKURA B. J.,
NAKAI S.
Publication year - 1986
Publication title -
journal of food biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.507
H-Index - 47
eISSN - 1745-4514
pISSN - 0145-8884
DOI - 10.1111/j.1745-4514.1986.tb00093.x
Subject(s) - linearization , reliability (semiconductor) , michaelis–menten kinetics , order of reaction , mathematics , experimental data , reaction conditions , simple (philosophy) , computer science , chemistry , nonlinear system , thermodynamics , statistics , reaction rate constant , kinetics , physics , organic chemistry , quantum mechanics , enzyme assay , enzyme , power (physics) , philosophy , epistemology , catalysis
A simple Basic program was tested with the aid of mathematical models for its reliability for the determination of reaction orders of chemical and enzymatic reactions. Accurate determination of reaction order in hypothetical models was achieved with 5–10 data points, provided that data were available for approximately 80% completion of the reaction. If the maximum extent of completion of the reaction was 60%, then even 20 data points were insufficient for accurate determination. The procedure was also combined with a linearization method and applied to experimental data for the determination of Michaelis‐Menten parameters. Mathematically derived V 0 's (initial velocities) allowed calculation of K m and V mx with reduced standard errors.