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CHARACTERIZATION OF [ 14 C]‐STARCH DEGRADATION BY RHIZOPUS GLUCOAMYLASE
Author(s) -
MEYERS MARC A.,
WASSERMAN BRUCE P.
Publication year - 1985
Publication title -
journal of food biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.507
H-Index - 47
eISSN - 1745-4514
pISSN - 0145-8884
DOI - 10.1111/j.1745-4514.1985.tb00351.x
Subject(s) - canna , chemistry , starch , pullulanase , size exclusion chromatography , hydrolysis , rhizopus , chromatography , thin layer chromatography , elution , paper chromatography , enzyme , biochemistry , fermentation
The hydrolysis of [ 14 C]‐labelled tobacco starch by Rhizopus niveus glucoamylase was investigated using thin‐layer and gel filtration chromatography. Hydrolysis was characterized by a period of rapid [ 14 C]‐glucose release, representing 15–30% of the total radioactivity, followed by a prolonged period of slow [ 14 C]‐glucose release. Pullulanase enhanced [ 14 C]‐glucose release, suggesting the presence of α‐(1→6) branch points. Thin‐layer chromatography was used as a rapid screening method for detecting glucoamylase activity in fractions eluted from a DEAE column of a Thermomyces lanuginosus culture filtrate, and gave results comparable to a coupled enzymatic method. [ 14 C]‐Labelled starch from Canna leaf was less susceptible to amylolytic digestion than [ 14 C]‐labelled tobacco starch.