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SCREENING FOR PECTINOLYTIC CANDIDA YEASTS: OPTIMIZATION AND CHARACTERIZATION OF THE ENZYMES
Author(s) -
CALL H. P.,
HARDING M.,
EMEIS C. C.
Publication year - 1985
Publication title -
journal of food biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.507
H-Index - 47
eISSN - 1745-4514
pISSN - 0145-8884
DOI - 10.1111/j.1745-4514.1985.tb00349.x
Subject(s) - pectinase , enzyme , maceration (sewage) , candida krusei , anaerobic exercise , chemistry , food science , enzyme assay , biochemistry , yeast , substrate (aquarium) , inulin , biology , candida tropicalis , ecology , physiology , materials science , composite material
In screening 72 Candida strains for extracellular enzymes, one acidic and seven alkaline proteases, one amylase and six pectinases were found. Candida kefyr, Candida macedoniensis and four strains from Candida pseudotropicalis were pectinolytically active; Candida kefyr showed considerable activity also under aerobic conditions but the highest activities were attained under strictly anaerobic conditions with N 2 gassing.YNB and peptone were the best nitrogen sources as regards enzyme production. The highest enzyme activities were achieved with succinic acid (aerobic) and inulin (anaerobic) as carbon sources. Enzyme production under aerobic conditions was considerably increased at pH 3.0. The addition of inducers (pectins or pectin ballast substances) led only to very slightly increased enzyme production.From kinetic studies of the enzyme, optimum activity was found to lie at pH 5.0 and 50°C. Subsequent characterization of the enzyme showed it to be an endo‐polygalacturonase.Eight to ten bands could be resolved by analytical isoelectric focussing. The K m value was found to be 1.14 × 10 −5 mol. The maceration test, using potato, carrot and apple tissue as substrate, showed considerable maceration activity, especially in the case of apple.