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ELECTROPHORESIS OF A PENICILLIUM DEXTRANASE USING POLYACRYLAMIDE GEL WITH BLUE DEXTRAN
Author(s) -
CALL H.P.,
FELDNER J.,
EMEIS C.C.
Publication year - 1983
Publication title -
journal of food biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.507
H-Index - 47
eISSN - 1745-4514
pISSN - 0145-8884
DOI - 10.1111/j.1745-4514.1983.tb00791.x
Subject(s) - dextranase , dextran , substrate (aquarium) , polyacrylamide gel electrophoresis , chromatography , chemistry , incubation , enzyme , electrophoresis , polyacrylamide , enzyme assay , penicillium , biochemistry , biology , food science , polymer chemistry , ecology
A commercial dextranase of Penicillium species was separated by gel‐electrophoresis, using included dextran blue as the enzyme substrate . Subsequent incubation at the pH of optimal activity (6) and. at 37°C rendered visible a total of 14 enzyme components produced by degradation of the stained substrate at the site of enzyme activity .