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BLUE PECTIN: A NEW SUBSTRATE FOR DETERMINING PECTINOLYTIC ACTIVITY
Author(s) -
CALL H.P.,
EMEIS C.C.
Publication year - 1983
Publication title -
journal of food biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.507
H-Index - 47
eISSN - 1745-4514
pISSN - 0145-8884
DOI - 10.1111/j.1745-4514.1983.tb00082.x
Subject(s) - substrate (aquarium) , pectin , agar , chromatography , enzyme , chemistry , food science , biochemistry , bacteria , biology , ecology , genetics
A new method of determining pectinolytic activities was developed with the aid of dye‐substituted substrate. Soluble stained pectic acid proved to be the most suitable substrate for the agar diffusion, the cupplate and the inoculation test, especially for screening purposes. Insoluble stained pectic acid was used as an enzyme substrate in the photometric test. The sensitivity of the method of 2 × 10 ‐2 μmol of reducing groups liberated per min per ml of enzyme may be considered satisfactory.