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CONVERSION OF EXOGENOUS CYTOKININS TO BIOLOGICALLY ACTIVE COMPOUNDS IN TOMATO TISSUE. EVIDENCE FOR AN ENZYME MEDIATED REACTION 1
Author(s) -
MARSHALL MAURICE R.,
CHISM GRADY W.
Publication year - 1982
Publication title -
journal of food biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.507
H-Index - 47
eISSN - 1745-4514
pISSN - 0145-8884
DOI - 10.1111/j.1745-4514.1982.tb00683.x
Subject(s) - enzyme , biochemistry , chemistry , biological activity , enzyme assay , combinatorial chemistry , in vitro
High performance liquid chromatography was used to follow the conversion of isopentenyladenine (i 6 Ade) in tomato pericarp cubes. Exogenously supplied i 6 Ade introduced by vacuum infiltration into tomato cubes (Lycopersicon esculentum, Mill “Rutgers”) declined 60%, when the cubes were incubated 2 h. The rate of i 6 Ade disappearance declined as the fruit ripened from mature green to a ripe fruit. Adenosine infiltrated with i 6 Ade did not alter the rate of i 6 Ade disappearance. However, adenine at the same concentration as adenosine inhibited (80%) the rate of i 6 Ade disappearance. Dinitrophenol (DNP) infiltrated 1 h prior to i 6 Ade infiltration inhibited completely the rate of i 6 Ade disappearance. However, DNP infiltrated simultaneously with i 6 Ade caused only partial inhibition (60%) of i 6 Ade disappearance. Inhibition by DNP could be overcome by increasing the concentration of i 6 Ade in the infiltrating medium. Tissue culture bioassay showed that i 6 Ade was converted to a biologically active form with greater activity than i 6 Ade.