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PROTEIN‐PHYTATE INTERACTIONS IN SOYBEANS. I. LOCALIZATION OF PHYTATE IN PROTEIN BODIES AND GLOBOIDS
Author(s) -
PRATTLEY C. A.,
STANLEY D. W.
Publication year - 1982
Publication title -
journal of food biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.507
H-Index - 47
eISSN - 1745-4514
pISSN - 0145-8884
DOI - 10.1111/j.1745-4514.1982.tb00305.x
Subject(s) - phytic acid , chemistry , calcium , soy protein , size exclusion chromatography , centrifugation , biochemistry , phosphorus , fraction (chemistry) , food science , soybean proteins , plant protein , chromatography , enzyme , organic chemistry
A study was made of whole soybeans, isolated protein bodies and a fraction consisting of globoid inclusions separated from protein bodies by centrifugation in order to observe the cellular location of phytate and to determine any indication of protein‐phytate interaction within the soybean cell. Microstructural and chemical evidence demonstrated phytic acid to be located in the protein bodies of the soybean, likely in the form of a soluble protein‐phytate salt with significant amounts specifically deposited in globoid inclusions. The probable existence of a protein‐cation‐phytate complex in situ was shown by gel filtration in which calcium and phosphorus was found to coelute with soluble proteins. The proteins involved in this interaction were tentatively identified as the 7S soybean fraction.