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OPTIMIZATION OF NUTRIENTS AND CULTIVATION CONDITIONS IN GLUCOAMYLASE PRODUCTION
Author(s) -
LEWIS NORMAN F.,
SINKAR VILAS P.
Publication year - 1981
Publication title -
journal of food biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.507
H-Index - 47
eISSN - 1745-4514
pISSN - 0145-8884
DOI - 10.1111/j.1745-4514.1981.tb00662.x
Subject(s) - incubation , aspergillus niger , yield (engineering) , nutrient , biomass (ecology) , chemistry , food science , strain (injury) , growth rate , enzyme , incubation period , biochemistry , biology , agronomy , mathematics , materials science , organic chemistry , geometry , anatomy , metallurgy
Initial adjustment of pH of growth medium in the range of 3.0–5.0 was found to be necessary for optimal production of glucoamylase by a newly isolated strain of Aspergillus niger. Optimum temperature of incubation under stationary conditions was found to be 26–28° C, although incubation at higher temperatures viz. 30 and 37° C led to faster fungal growth rate and increased biomass. In complex medium, the optimum glucose and peptone concentrations were 2 and 3% respectively, and the presence of trace elements was found to be essential for maximal enzyme yield.