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MODIFICATION OF THE AGRANOFF‐SUOMI METHOD FOR THE SYNTHESIS OF CDP‐DIACYLGLYCEROL
Author(s) -
CARMAN GEORGE M.,
FISCHL ANTHONY S.
Publication year - 1980
Publication title -
journal of food biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.507
H-Index - 47
eISSN - 1745-4514
pISSN - 0145-8884
DOI - 10.1111/j.1745-4514.1980.tb00877.x
Subject(s) - diacylglycerol kinase , phosphatidylserine , atp synthase , chemistry , enzyme , chloroform , phosphatidylinositol , pyridine , biochemistry , inositol , biosynthesis , chromatography , glycerol , organic chemistry , phospholipid , protein kinase c , membrane , receptor , kinase
Cytidine 5′‐diphospho‐1,2‐diacyl‐sn‐glycerol (CDP‐diacylglycerol) is an important liponucleotide intermediate in the biosynthesis of phosphatidyl‐inositol, phosphatidylserine and phosphatidylglycerophosphate. The Agranoff‐Suomi method (Agranoff, B. W. and Suomi, W. D. (1963) Biochem. Prep. 10, 47–51) for the synthesis of CDP‐diacylglycerol was modified by a procedure which eliminates the lyophilization step required for the preparation of reactants. The reaction was run in chloroform instead of pyridine using the catalyst 4‐dimethylaminopyridine. The product of the reaction was purified by silica gel column chromatography and is enzymatically active with the enzymes phosphatidylinositol synthase, phosphatidylserine synthase and phosphatidylglycerophosphate synthase.