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THE ROLE OF AMINO GROUPS IN THE BIOLOGICAL AND ANTIGENIC ACTIVITIES OF CLOSTRIDIUM PERFRINGENS TYPE A ENTEROTOXIN
Author(s) -
WHITAKER JOHN R.,
GRANUM PER EINAR
Publication year - 1980
Publication title -
journal of food biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.507
H-Index - 47
eISSN - 1745-4514
pISSN - 0145-8884
DOI - 10.1111/j.1745-4514.1980.tb00781.x
Subject(s) - enterotoxin , dtnb , chemistry , clostridium perfringens , sodium dodecyl sulfate , guanidine , amino acid , phosphate , biochemistry , microbiology and biotechnology , chromatography , bacteria , escherichia coli , biology , enzyme , glutathione , genetics , gene
Clostridium perfingens type A enterotoxin has 8 amino groups which are readily reactive with 2, 4, 6‐trinitrobenzene sulfonic acid (TNBS) in 20 mM phosphate buffer, pH 6.8, and 10 amino groups which react with TNBS only after denaturation with 6.5 M guanidine · HCl ≤ 25°C. The one sulfhydryl group of enterotoxin is not reactive with 5,5′‐dithiobis‐(2‐nitrobenzoic acid) (DTNB) in the native molecule (phosphate buffer, pH 6.8), but is reactive in the presence of 0.2% sodium dodecyl sulfate at 25°C. i