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EXTRACTION OF PROTEINS FROM BUCKWHEAT BRAN
Author(s) -
GROSSMAN MARIA V.,
RAO CHIGURUPATI S.,
DA SILVA RUI SERGIO F.
Publication year - 1980
Publication title -
journal of food biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.507
H-Index - 47
eISSN - 1745-4514
pISSN - 0145-8884
DOI - 10.1111/j.1745-4514.1980.tb00656.x
Subject(s) - bran , pectinase , cellulase , extraction (chemistry) , yield (engineering) , chemistry , food science , chromatography , enzyme , biochemistry , raw material , organic chemistry , materials science , metallurgy
Proteins from buckwheat bran were extracted by a wet alkaline extraction process, giving a yield of 37.0% of the total nitrogen in the liquid extract, at pH of 6.5. However, the yield could be improved, given a pretreatment of the bran with carbohydrases, thus producing a yield of 56% with cellulase , 58% with pectinase, whereas the increase was minimal with hemicellulase. The maximum yield of 67.5% was obtained by synergistic action of pectinase and hemicellulase, in a pretreatment lasting for 7 h at pH of 3.7.