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KINETIC PROPERTIES OF PHOSPHATIDYLINOSITOL SYNTHASE FROM GERMINATING SOYBEANS
Author(s) -
CARMAN GEORGE M.,
DOUGHERTY MAUREEN
Publication year - 1980
Publication title -
journal of food biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.507
H-Index - 47
eISSN - 1745-4514
pISSN - 0145-8884
DOI - 10.1111/j.1745-4514.1980.tb00652.x
Subject(s) - diacylglycerol kinase , phosphatidylinositol , microsome , inositol , chemistry , biochemistry , glycerol , diglyceride , enzyme , substrate (aquarium) , pi , biology , protein kinase c , signal transduction , receptor , ecology
CDP ‐1,2‐ diacyl ‐sn‐ glycerol (CDP‐diacylglycerol): myo‐inositol phosphatidyltransferase (EC. 2.7.8.11) catalyzes the final step in the de novo synthesis of phosphatidylinositol in the microsome fraction of germinating soybeans. The Michaelis constants for CDP‐diacylglycerol and myo‐inositol were determined for the reaction using a Triton X‐100‐CDP‐diacylglycerol mixed micelle substrate. The K m values for CDP‐diacylglycerol and myo‐inositol were 30μ M and 0.11 mM, respectively.