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IRRADIATION OF INDUSTRIAL ENZYME PREPARATIONS II. CHARACTERIZATION OF FUNGAL PECTINASE BY THIN‐LAYER ISOELECTRIC FOCUSING AND GEL FILTRATION
Author(s) -
DELINĆEE HENRY
Publication year - 1978
Publication title -
journal of food biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.507
H-Index - 47
eISSN - 1745-4514
pISSN - 0145-8884
DOI - 10.1111/j.1745-4514.1978.tb00604.x
Subject(s) - isoelectric focusing , pectinase , pectinesterase , isoelectric point , chemistry , size exclusion chromatography , chromatography , pectin , biochemistry , enzyme
ABSTRACT Industrial dry fungal pectinase from A. niger was irradiated with doses (up to 1 Mrad) of 60 Co‐γrays effective in reducing microbial contamination. The pectinase was characterized by thin‐layer isoelectric focusing and gel filtration in order to detect possible radiation‐induced structural alterations. Thin‐layer isoelectric focusing revealed at least fifteen multiple forms with pectin‐depolymerizing activity, with isoelectric points in the range pH 4.5–7. Heterogeneity of pectinesterase was also demonstrated, the main band occurring around pH 4. By thin‐layer gel filtration the molecular weight of the pectin‐depolymerase was estimated as being about 36,000, and that of pectinesterase as about 33,000. Radiation‐induced changes of the charge properties or molecular size of the irradiated pectinase preparation were not observed. The feasibility of using ionizing radiation for the reduction of microbial contamination of industrial enzyme preparations looks promising.