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A SIMPLIFIED METHOD FOR ACCURATE DETERMINATION OF CELL WALL URONIDE CONTENT
Author(s) -
AHMED AHMED EL RAYAH,
LABAVITCH JOHN M.
Publication year - 1978
Publication title -
journal of food biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.507
H-Index - 47
eISSN - 1745-4514
pISSN - 0145-8884
DOI - 10.1111/j.1745-4514.1978.tb00193.x
Subject(s) - uronic acid , sulfuric acid , chemistry , chromatography , cell wall , dissolution , polysaccharide , biochemistry , organic chemistry
Reproducible determinations of cell wall uronic acid content are obtained by first dissolving wall samples in sulfuric acid and then performing assays on aliquots of the cell wall solution. The colorimetric assay utilizing m‐hydroxydiphenyl is recommended because of its low background readings in the presence of large quantities of neutral sugars.