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A PURIFIED POLYGALACTURONASE FOR CELL WALL ANALYSIS
Author(s) -
LABAVITCH JOHN M.,
RAE HENRY L.
Publication year - 1978
Publication title -
journal of food biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.507
H-Index - 47
eISSN - 1745-4514
pISSN - 0145-8884
DOI - 10.1111/j.1745-4514.1978.tb00188.x
Subject(s) - pectinase , aspergillus niger , glycosidic bond , cell wall , biochemistry , chemistry , enzyme , chromatography
An endopolygalacturonase has been purified from a crude commercial preparation that is derived from cultures of Aspergillus niger. The polygalacturonase has a pH optimum of 4.5 and is relatively heat labile, losing activity when heated to 45°C. The purified preparation acts on the α‐1, 4‐galacturonosyl linkages present in polygalacturonic acid. It does not act on linkages present in a variety of substrates which contain virtually all of the glycosidic bond types described for the cell walls of monocots, dicots, and various fruits.