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Increased Plasma Angiogenin Level is Associated and May Contribute to Decreased T‐Cell Zeta‐Chain Expression in Hemodialysis Patients
Author(s) -
Eleftheriadis Theodoros,
Kartsios Charalambos,
Pissas Georgios,
Liakopoulos Vassilios,
Antoniadi Georgia,
Galaktidou Grammati,
Stefanidis Ioannis
Publication year - 2013
Publication title -
therapeutic apheresis and dialysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.415
H-Index - 53
eISSN - 1744-9987
pISSN - 1744-9979
DOI - 10.1111/j.1744-9987.2012.01135.x
Subject(s) - angiogenin , medicine , downregulation and upregulation , flow cytometry , cell , microbiology and biotechnology , immunology , endocrinology , andrology , biology , biochemistry , angiogenesis , gene
Hemodialysis (HD) patients are characterized by impaired T‐cell function at least in part because of T‐cell zeta‐chain downregulation due to inflammation. Angiogenin responds as an acute phase reactant, is increased in HD patients, suppresses T‐cell function and increased angiogenin level is co‐localized with T‐cell zeta‐chain downregulation in various pathologies. Angiogenin can inhibit translation of proteins, which lack internal ribosomal entry sites in the corresponding mRNAs. In this study, the possible effect of angiogenin on T‐cell zeta‐chain downregulation was evaluated. Thirty HD patients and 21 healthy volunteers participated. T‐cell zeta‐chain expression was assessed with flow cytometry, plasma angiogenin and serum IL‐6 with ELISA and serum C‐reactive protein with an immunoturbidimetric method. Two available software tools were used for predicting the presence or not of internal ribosomal entry sites in T‐cell zeta‐chain mRNA sequence. In silico analysis of T‐cell zeta‐chain mRNA sequence failed to reveal the presence of internal ribosomal entry sites. T‐cell zeta‐chain expression was lower in HD patients than in healthy volunteers (1.86 ± 0.63 vs. 4.73 ± 3.22). In HD patients, C‐reactive protein as well as IL‐6 were higher than in healthy volunteers (10.04 ± 15.13 mg/L vs. 3.43 ± 0.98 mg/L and 15.06 ± 13.08pg/mL vs. 2.11 ± 2.10 pg/mL respectively). Angiogenin was higher in HD patients than in healthy volunteers (483.20 ± 154.07 ng/mL vs. 259.98 ± 64.15 ng/mL). Neither C‐reactive protein, nor IL‐6 was associated with angiogenin or T‐cell zeta‐chain expression. Angiogenin concentration was negatively related to the expression of T‐cell zeta‐chain (r = −0.410, P = 0.025). Increased angiogenin may contribute to decreased T‐cell zeta‐chain expression in HD patients.