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Establishment of a Blood Purification System for Renal Failure Rats Using Small‐Size Dialyzer Membranes
Author(s) -
Yorimitsu Daisuke,
Satoh Minoru,
Koremoto Masahide,
Haruna Yoshisuke,
Nagasu Hajime,
Kuwabara Atsunori,
Sasaki Tamaki,
Kashihara Naoki
Publication year - 2012
Publication title -
therapeutic apheresis and dialysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.415
H-Index - 53
eISSN - 1744-9987
pISSN - 1744-9979
DOI - 10.1111/j.1744-9987.2012.01091.x
Subject(s) - medicine , hemodialysis , dialysis , blood urea nitrogen , creatinine , extracorporeal circulation , blood flow , renal blood flow , heart rate , urology , renal function , blood pressure
Hemodialysis techniques for small animals have not been established because no small dialysis apparatus has been available. We recently developed a small‐size dialyzer and established an appropriate blood purification system for small animals. To confirm the appropriate dialysate flow rate, bovine blood was dialyzed for 60 min at a fixed blood flow rate of 1.0 mL/min and variable dialysate flow rates. Blood urea nitrogen and creatinine levels decreased significantly at a dialysate flow rate of 5 mL/min (from 13.7 ± 0.2 to 10.3 ± 1.2 mg/dL and 1.07 ± 0.15 to 0.61 ± 0.12 mg/dL, respectively, P < 0.05). To determine the appropriate in vivo conditions, extracorporeal circulation was performed in anesthetized male Sprague‐Dawley rats at a dialysate flow rate of 0.0 mL/min, for 240 min, and at variable blood flow rates. Extracorporeal circulation was successful at a blood flow rate of 1.0 mL/min, but not 1.5 mL/min. To establish in vivo hemodialysis conditions, we used the animal model of end stage renal failure. Sprague‐Dawley rats were fed a 0.75% adenine‐containing diet for 3 weeks, after which they received hemodialysis for 120 min at a dialysate and blood flow rate of 5.0 and 1.0 mL/min, respectively. There were no significant changes in systolic blood pressure or heart rate during dialysis. Thus, this blood purification system can be safely used for small animals at a dialysate flow rate of 5.0 mL/min and a blood flow rate of 1.0 mL/min. This system provides a basis for further research on hemodialysis therapy.