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Preparation of Cu 2+ ‐loaded Porous Chitosan Particles for Immunoglobulin G Adsorption
Author(s) -
Yue RongZheng,
Liu Ting,
Su BaiHai,
Fu Ping,
Zhao Fan,
Zhao ChangSheng
Publication year - 2008
Publication title -
therapeutic apheresis and dialysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.415
H-Index - 53
eISSN - 1744-9987
pISSN - 1744-9979
DOI - 10.1111/j.1744-9987.2008.00576.x
Subject(s) - adsorption , chitosan , porosity , sorbent , aqueous solution , particle (ecology) , chemical engineering , chromatography , particle size , precipitation , immunoglobulin g , albumin , materials science , medicine , antibody , chemistry , composite material , organic chemistry , immunology , oceanography , physics , meteorology , engineering , geology
Chitosan porous particles were prepared using a precipitation technique. The porous particles could bind Cu 2+ , from which Cu 2+ ‐loaded porous particles were prepared. The Cu 2+ ‐loaded porous chitosan particles could remove immunoglobulin (Ig) G more selectively than albumin, IgA and IgM from aqueous solutions and human plasma. The effect of the volume ratio of plasma to particles on protein adsorption was studied, with the results indicating that a volume ratio of 3:1 might be a good choice for clinical use. The particles could be easily incorporated into a column. When human plasma was applied to the particle column, higher removal efficiency was obtained. These results suggested that Cu 2+ ‐loaded porous particles may be a potentially good sorbent for IgG removal from plasma.