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Molecular identification and characterization of the Orco orthologue of Spodoptera litura
Author(s) -
Wu ZhongNan,
Chen Xi,
Du YongJun,
Zhou JingJiang,
ZhuGe QiChuan
Publication year - 2013
Publication title -
insect science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.991
H-Index - 45
eISSN - 1744-7917
pISSN - 1672-9609
DOI - 10.1111/j.1744-7917.2011.01483.x
Subject(s) - biology , spodoptera litura , subfamily , transmembrane protein , transmembrane domain , identification (biology) , insect , extracellular , amino acid , genetics , microbiology and biotechnology , gene , botany , receptor , larva
A highly conserved and broadly expressed receptor protein Orco (olfactory coreceptor) is crucial for insect olfaction, and an orthologue of Orco has been identified in several insect species. Here we report the identification and characterization of Orco from Spodoptera litura . The protein displays high primary amino acid sequence conservation with other previously identified Orco orthologues. Bioinformatic analysis revealed that it has common features with other members of the Orco subfamily: seven‐transmembrane domains with intracellular N‐terminus and extracellular C‐terminus. The transcript was detected in abundance in the chemosensory organs of the antennae of both male and female adults by real‐time polymerase chain reaction analysis, and was localized at the bases of all categories of olfactory sensilla through in situ hybridization.