Premium
A cost‐effective, simple and high‐throughput method for DNA extraction from insects
Author(s) -
Lagisz Malgorzata,
Port Gordon,
Wolff Kirsten
Publication year - 2010
Publication title -
insect science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.991
H-Index - 45
eISSN - 1744-7917
pISSN - 1672-9609
DOI - 10.1111/j.1744-7917.2010.01318.x
Subject(s) - consumables , biology , dna extraction , extraction (chemistry) , polymerase chain reaction , throughput , dna , protocol (science) , biochemical engineering , microbiology and biotechnology , computational biology , process engineering , chromatography , biological system , computer science , engineering , genetics , gene , telecommunications , pathology , business , wireless , medicine , chemistry , alternative medicine , marketing
We present a high‐throughput cost‐effective method to extract DNA suitable for polymerase chain reaction (PCR) from insect tissue. The method uses standard 200 μL‐deep 96‐well plates in which samples are ground, digested and subsequently purified. The test extraction using four different insect species and controlling for potential contamination showed that the method yields good‐quantity and quality DNA. PCR with mitochondrial and nuclear primers was reliable. The proposed extraction protocol combines the speed of commercial 96‐well plate methods with the economies associated with readily available and cheap laboratory chemicals, consumables and equipment. Therefore, this method is particularly suitable for low‐budget research projects and for laboratories with only basic equipment present.