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Cloning and characterization of the gene encoding an ubiquitin‐activating enzyme E1 domain‐containing protein of silkworm, Bombyx mori
Author(s) -
Wu Ping,
Li MuWang,
Jiang YunFeng,
Wang ZiSheng,
Guo XiJie
Publication year - 2010
Publication title -
insect science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.991
H-Index - 45
eISSN - 1744-7917
pISSN - 1672-9609
DOI - 10.1111/j.1744-7917.2009.01304.x
Subject(s) - bombyx mori , biology , open reading frame , midgut , gene , complementary dna , microbiology and biotechnology , untranslated region , rapid amplification of cdna ends , genetics , peptide sequence , messenger rna , botany , larva
Bombyx mori cytoplasmic polyhedrosis virus (BmCPV) is one of the major viral pathogens for the silkworm. To date, the molecular mechanism of BmCPV invasion has been unclear. We cloned the full length complementary (c)DNA which encodes the ubiquitin‐activating enzyme E1‐domain containing protein1 (UbE1DC1) of Bombyx mori by using suppression subtractive hybridization (SSH) and rapid amplification of complementary (c)DNA ends (RACE). The full‐length cDNA of UbE1DC1 gene is 1 919 bp, consisting of a 100 bp 5′ untranslated region, a 637 bp 3′ untranslated region and an 1 182 bp open reading frame (ORF), encoding a 393 amino acid protein. The protein contained the THiF_MoeB_hesA_family domain, an adenosine triphosphate binding site, which belongs to the family of ubiquitin‐activating enzyme E1. Reverse transcription – polymerase chain reaction analysis from the silkworm tissues, namely silk gland, hemocyte, fat body, gonad and midgut revealed that UbE1DC1 was expressed in all the five tissues. The real‐time quantitative polymerase chain reaction analysis indicated that the relative expression of UbE1DC1 in the normal midgut was approximately 9.78‐fold of that in the BmCPV‐infected midgut. It is implicated that UbE1DC1 may play an important role in the interaction between the host and BmCPV invasion.